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Grace HPLC Columns
Grace HPLC Columns
Grace is known for high quality HPLC columns for the pharmaceutical and life science industries. Formerly, Vydac, Alltima and Vision HT HPLC columns were supplied by Grace. For some time now, these columns have been produced by Avantor. Some more of the former Grace HPLC columns are now produced by Dr. Maisch. As usual, at Analytics-Shop you can obtain all columns from a single source.
Packing: Alltima HP, GraceSmart, Prevail, Vision HT, etc.
If you have any questions concerning products of Grace, please contact our technical support team.
Alltima HP
AlltimaTM HP columns combine Grace´s best phase chemistries with high-purity silica. The result is one product family with the selectivity and performance needed to help overcome your most challenging separation needs. The column´s most important properties are:
• Better peak symmetry—high-purity silica eliminates peak tailing problems
• Long column life—exceptional column stability minimizes downtime and reduces cost
• Ideal for microbore or critical analysis—low to no detectable column bleed
• Multiple selectivity options—optimizes retention, resolution and analysis time
| Phase |
Particle Size in µm |
Pore Size in Å |
% Carbon Load |
Endcapping |
| C18 | 3, 5 | 190 | 12 | Yes |
| C18 EPS | 3, 5 | 190 | 4 | No |
| C18 Hi-Load | 3, 5 | 100 | 24 | Yes |
| C18 AQ | 3, 5 | 100 | 20 | Yes |
| C18 Amide | 3, 5 | 190 | 12 | Yes |
| C8 | 3, 5 | 190 | 8 | Yes |
| Cyano | 3, 5 | 190 | 4 | Yes |
| Silica | 3, 5 | 100 | - | No |
| Hilic | 1.5, 3, 5 | 120 | - | No |
GraceSmart
GraceSmartTM HPLC phases use high purity silica and are monomerically bonded with uniform coverage. This translates into symmetrical peaks for acids/bases and predictable reversed-phase selectivity. Whether for routine analysis or new method development, use GraceSmartTM columns to get premium performance at an exceptional value. The most important properties are:
• High purity phases
• Efficient and reproducible separations
• Classic reversed-phase selectivity
| Phase |
Particle Size in µm |
Pore Size in Å |
% Carbon Load |
Endcapping |
| C18 | 3, 5 | 120 | 10 | Ja |
Prevail
PrevailTM HPLC columns offer exceptional versatility for difficult separations. Traditional reversed-phase packings experience bonded phase collapse under highly aqueous (>95%) conditions. PrevailTM bonded phases remain fully extended in 100% aqueous and offer a high carbon load for exceptional retention at 100% organic. Retain both polar and non- polar compounds simply by adjusting the mobile phase. The most important properties are:
• Exceptional bonded phase stability for long column life
• Excellent sensitivity with microbore and ELSD applications
| Phase |
Particle Size in µm |
Pore Size in Å |
% Carbon Load |
Endcapping |
| C18 Select | 3, 5 | 110 | 17 | Yes |
| C18 | 3, 5 | 110 | 15 | Yes |
| C8 | 3, 5 | 110 | 8 | Yes |
| Phenyl | 3, 5 | 110 | 7 | Yes |
| Cyano | 3, 5 | 110 | - | Yes |
| Amino | 3, 5 | 110 | - | No |
| Silica | 3, 5 | 110 | - | No |
| Carbohydrate ES | 5 | - | - | No |
Vision HT
Separate complex samples with excellent resolution with VisionHTTM high performance columns. They are available with 1.5, 3, 5 and 10μm particle sizes and six different phase selectivities spanning the full polarity spectrum. The same base silica and bonding chemistries are used across all particle sizes therefore offering simple method transfer between UHPLC, HPLC and preparative systems. The most important properties are:
• Easy method transfer between UHPLC, HPLC and preparative chromatography
• Broad selectivity range—unique complementary phase chemistries
• Maximize speed and resolution with sub 2μm on any LC system
| Phase |
Particle Size in µm |
Pore Size in Å |
% Carbon Load |
Endcapping |
| C18 classic | 1.5 | 100 | 6 | Yes |
| C18 basic | 1.5 | 120 | 5.5 | proprietary |
| C18 polar | 1.5 | 100 | 5 | No |
| C18 high load | 1.5 | 120 | 11 | proprietary |
| Hilic | 1.5 | 120 | No | |
| Silica | 1.5 | 120 | No |
Vydac MS
Vydac® MS columns are the latest development in the ongoing effort to provide the best reversed-phase HPLC columns for biomolecules. A proprietary surface treatment and bonding process give Vydac® MS columns truly unique selectivity. A variety of reversed-phases makes this product line suitable for small peptides to large intact, undenatured proteins. The most important properties are:
• Unique selectivity reveals peaks otherwise masked by other C18 columns
• Excellent peak shape with little or no TFA
• High protein recoveries make scale-up easy
| Phase |
Particle Size in µm |
Pore Size in Å |
% Carbon Load |
Endcapping |
| 208 MS C8 | 5 | 300 | 5 | Yes |
| 214 MS C4 | 5 | 300 | 3 | Yes |
| 218 MS C18 | 3, 5, 10, 10-15 | 300 | 8 | Yes |
| 238 MS C18 | 5 | 300 | 4 | Yes |
Vydac TP
The 300Å pores of TP silica provides polypeptides complete access to the interior of the silica. The unique process by which Grace manufactures Vydac® TP silica results in high purity with carefully controlled characteristics. It is the standard that has defined large pore HPLC silica for polypeptide separations for nearly two decades. The most important properties are:
• Exceptionally long column lifetime and negligible phase leaching
• Reliable protein purifications, scalable from analytical to preparative scale
| Phase |
Particle Size in µm |
Pore Size in Å |
% Carbon Load |
Endcapping |
| 201 TP C18 | 5, 7, 10, 10-15, 15-20 |
300 | 8 | No |
| 202 TP C18 | 3, 5, 10 |
300 | 9 | No |
| 208 TP C8 | 3, 5, 7, 10, 10-15, 15-20 |
300 | 5 | Yes |
| 214 TP C4 | 3, 5, 7, 10, 10-15, 15-20 |
300 | 3 | Yes |
| 218 TP C18 | 3, 5, 7, 10, 10-15, 15-20 |
300 | 8 | Yes |
| 219 TP Diphenyl | 3, 5, 7, 10, 10-15, 15-20 |
300 | 4 | Yes |
| 238 TP C18 | 3, 5, 7, 10, 10-15, 15-20 |
300 | 4 | Yes |