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Sielc HPLC Columns
SIELC HPLC Columns
SIELC is well-known for its high level of know-how in Chromatography and offers high quality columns. The Coresep series offers HILIC / ion exchange columns based on core-shell particles, but has been transferred to HelixChromatography. A high level of performance is guaranteed with a high flow rate and low back pressure. A wide range of normal and reverse phase columns in various lengths and diameters is also available with Primesep or Promix.
The technology of the SHARC columns (specific hydrogen-bond adsorption resolution column) is characterized by the predominant formation of hydrogen bonds, which focuses on the bonds to electronegative atoms. The columns from the SIELC Obelisc series have been designed for the separation of small molecules. The bonds here are based on the liquid separation cell technology (LiSC). Legacy columns are made of octadecylsilane bound to porous silica or ceramic particles which comply with the USP regulations.
Sielc SHARC HPLC Columns:
SHARC ™ HPLC columns are the latest innovation from SIELC Technologies and represent the first columns in which the separation is mainly based on hydrogen bonds. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Chromatography (Specific hydrogen bonds adsorption dissolution chromatography). Hydrogen bonds are the interaction between a positively polarized hydrogen atom bound to an electronegative atom such as oxygen, nitrogen, and fluorine with a second electronegative atom.
All SHARC HPLC columns from SIELC are available in different versions:
| Inner diameter [mm] | 22, 10, 4.6, 3.2, 2.1, 1, 0.5, 0.25 |
| Length [mm] | 250, 150, 100, 50, 25, 10 |
| Particel Size [mm] | 10, 5, 3 |
| Pore Size [Å] | 100 |
Sielc PRIMSEP HPLC Columns:
Primesep A columns are reversed-phase analysis columns with embedded strongly acidic functional groups. These groups improve the retention of weak basic compounds by cation exchange mechanisms. All mobile phases are compatible with LC-MS as well as with preparative chromatography.
All SIELC Primsep A HPLC columns are available in different versions:
| Inner diameter [mm] | 22, 10, 4.6, 3.2, 2.1, 1, 0.5, 0.25 |
| Length [mm] | 250, 150, 100, 50, 25, 10 |
| Particel Size [mm] | 10, 5 |
| Pore Size [Å] | 100 |
Primesep AB is also a reversed phase column with embedded acidic and basic functional groups. These groups improve the retention of strongly acidic and strongly basic compounds by ion exchange mechanisms and thus allows the separation of these. All mobile phases are compatible with LC-MS as well as with preparative chromatography.
All Primsep AB HPLC columns from SIELC are available in different versions:
| Inner diameter [mm] | 22, 10, 4.6, 3.2, 2.1, 1 |
| Length [mm] | 250, 150, 100, 50, 25, 10 |
| Particel Size [mm] | 10, 5 |
| Pore Size [Å] | 100 |
Primesep C is a reversed phase column with embedded complex-forming groups. Retention times of positively charged compounds are improved by complex formation.
All SIELC Primsep C HPLC columns are available in different versions:
| Inner diameter [mm] | 22, 10, 4.6, 3.2, 2.1, 1, 0.5, 0.25 |
| Length [mm] | 250, 150, 100, 50, 25, 10 |
| Particel Size [mm] | 10, 5 |
| Pore Size [Å] | 100 |
Primesep N is a normal phase analysis column with embedded acidic groups (pKa approx. 5). This improves the retention times of basic compounds. Polar compounds are separated by the HILIC mechanism. All mobile phases are compatible with LC-MS and preparative chromatography.
All Primusp N HPLC columns from SIELC are available in different versions:
| Inner diameter [mm] | 22, 10, 4.6, 3.2, 2.1, 1, 0.5, 0.25 |
| Length [mm] | 250, 150, 100, 50, 25, 10 |
| Partikelgröße [mm] | 10, 5 |
| Porengröße [Å] | 300, 100 |
Primesep SB is a reverse phase analysis column with embedded strongly basic functional groups. The retention times of acidic compounds are improved by anion exchange mechanisms. All mobile phases are compatible with LC-MS and preparative chromatography (pH range in the range of 1.5 to 5).
All SIELC Primsep SB HPLC columns are available in different versions:
| Inner diameter [mm] | 22, 10, 4.6, 3.2, 2.1, 1, 0.5, 0.25 |
| Length [mm] | 250, 150, 100, 50, 25, 10 |
| Particel Size [mm] | 10, 5 |
| Pore Size [Å] | 100 |
Sielc OBELISC HPLC Columns:
Obelisc ™ HPLC columns are the latest innovative columns from SIELC Technologies. Obelisc columns are the first commercially available columns with Liquid Separation Cell Technology (LiSCTM). The LiSC technology is based on a chemical modification of the silica gel pores, which creates a liquid separation cell with its own charge properties, ionic strength and hydrophobic properties. Like a living cell, which is in equilibrium with the external environment, liquid separation cells exist in a constant equilibrium with the mobile phase.
Obelisc R is a reversed-phase analysis column, with non-polar hydrophobic side chains bounded to silica gel or a polymer. By additional ionic groups on a long hydrophobic chain, Obelisc R offers additional retention times for analytes which are not given with a conventional reversed-phase column.
| Inner diameter [mm] | 22, 10, 4.6, 3.2, 2.1, 1, 0.5, 0.25 |
| Length [mm] | 250, 150, 100, 50, 25, 10 |
| Particel Size [mm] | 10, 5 |
| Pore Size [Å] | 100 |
The Obelisc N HPLC column is particularly suitable for the separation of polar and charged analytes due to its polar properties. Through positively and negatively charged functional groups, it is possible to interact with diverse charged analytes and separate them from each other by different retention times.
All Obelisc N HPLC columns from SIELC are available in different versions:
| Inner diameter [mm] | 22, 10, 4.6, 3.2, 2.1, 1, 0.5, 0.25 |
| Length [mm] | 250, 150, 100, 50, 25, 10 |
| Particel Size [mm] | 10, 5 |
| Pore Size [Å] | 100 |