Waters is the leader in modern HPLC column technology and the pioneer of UPLC® columns. Waters' separation columns are characterised by their high batch-to-batch reproducibility, as Waters is one of only a few column manufacturers worldwide to produce the filling materials and columns itself. Thus, Waters columns belong to the standard equipment of every laboratory. The innovative columns lead to considerable time and cost savings per sample in their analysis processes, with better quality laboratory results. Select the desired configurations from over 5,000 Waters HPLC columns using the drop-down menu on the left.
Detailed information on selected Waters HPLC columns has been listed in the following section. If you require further information, please feel free to contact us.
Atlantis is the industry standard for polar compound retention for Reversed Phase and HILIC separations. Waters Atlantis HPLC columns are available with T3 (for enhanced retention of polar analytes), HILIC (for HILIC separations) and C18 (for 100 % aqueous mobile phases). Atlantis T3 columns are ideally suited for mixtures of very different analytes.
Phase | Particle Size (µm) |
Pore Size (Å) |
Carbon Load (%) |
Endcapping |
T3 | 3, 5, 10 | 100 | 14 | Yes |
C18 | 3, 5, 10 | 100 | 12 | Yes |
HILIC | 3, 5 | 100 |
SunFire HPLC columns are well suited for method development. These columns have a high sample loading capacity, especially for basic analytes in mobile phases with a low pH. They are mainly used to detect impurities in samples.
Phase | Particle Size (µm) |
Pore Size (Å) |
Carbon Load (%) |
Endcapping |
C18 | 2.5, 3.5, 5, 10 | 100 | 16 | Yes |
C8 | 2.5, 3.5, 5, 10 | 100 | 12 | Yes |
Silica | 5, 10 | 100 |
Due to their high pH stability of 1 – 12, Waters XBridge HPLC columns are typical allrounders. Hence, they can be used widely and are particularly suitable for method development.
Phase | Particle Size (µm) |
Pore Size (Å) |
Carbon Load (%) |
Endcapping |
C18 | 2.5, 3.5, 5, 10 | 130 | 18 | Yes |
C8 | 2.5, 3.5, 5, 10 | 130 | 13 | Yes |
Shield RP 18 | 2.5, 3.5, 5, 10 | 130 | 17 | Yes |
Phenyl | 2.5, 3.5, 5 | 130 | 15 | Yes |
HILIC | 2.5, 3.5, 5 | 130 | ||
Amide | 2.5, 3.5 | 130 | 12 |
Acquity columns from Waters have been developed and tested for use in applications up to 15,000 psi (1,000 bar). Unmatched in efficiency, robustness and throughput capacity, they enable the combination of faster separations with higher resolution by fully utilizing the potential of small particles.
Avoiding sample loss of metal-sensitive analytes: non-specific adsorption (NSA) of metal sensitive compounds can be challenging when using regular stainless steel columns. You can be confronted with long column passivation times, chromatography with large RSDs, and broad peaks that can be difficult to detect.
PREMIER columns have MaxPeak High Performance Surfaces (HPS) which effectively reduce NSA losses caused by metal interactions. Waters PREMIER HPLC columns provide reduced column passivation, increased reproducibility, enhanced sensitivity and improved peak shapes.
Waters ATLANTIS PREMIER HPLC columns and Waters ACQUITY PREMIER UPLC columns are available in the PREMIER configuration.