HPLC Column ProPac HIC-10, 300 Å, 5 µm, 2.1 x 100 mm

ProPac HIC-10 HPLC column with 2.1 mm inner diameter, 100 mm length and 5.0 µm particle size.

About ProPac HIC-10 Columns

With Thermo Scientific™ ProPac™ HIC-10-LC columns you can achieve high-resolution separation of proteins and peptides under non-denaturing conditions. These silica-based columns are designed with multifunctional ligands and offer exceptionally high hydrolytic stability under the highly aqueous conditions of HIC.

The HIC-10 column provides you with a high protein loading capacity for protein purification and a wide range of analytical and preparative applications. This includes the separation of proteins in bovine serum, proteins in snake venom and monoclonal antibodies (Mabs). The packing material is based on 5 µm ultra-high purity spherical silica particles with 300 Å pores.

About hydrophobic interaction chromatography (HIC)

Hydrophobic interaction chromatography separates biomolecules in a decreasing salt gradient based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion exchange and gel filtration chromatography.