HPLC Column Semi-Prep, ZORBAX GF-250, 4 µm, 9,4 x 250 mm

The Agilent ZORBAX GF-250 and GF-450 SEC (gel filtration) columns are designed for the size separation of proteins and other biomolecules. The separation range is 4,000-900,000 for globular proteins when GF-250 and GF-450 columns are used in series.

The GF-250/GF-450 SEC columns have a hydrophilic, diol-bound phase for high protein recovery (typically >90%) and a unique zirconia modification of the silica for a pH working range of 3-8. The HPLC column shown here has inner diameter 9.4 mm, length 250 mm and 4.0 µm large particles.

The result is a column that can be used for both analytical and preparative protein separations with flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation.

Typical applications include the separation of protein monomers, dimers and aggregates, desalting, protein molecular weight determination and separation of modified proteins.