Thermo Scientific HPLC Columns
ThermoFisher HPLC Columns
Here you will find an extensive range of HPLC columns from Thermo Scientific, with over 3,500 columns to choose from. Thermo Scientific, a part of Thermo Fisher Scientific, is known for its high-quality columns, especially for the classic Hypersil. Besides numerous other packing materials (Aquasil, Accucore, Hypersil, Hypersil BDS, HyPurity, etc.) you will also find the Acclaim columns from Dionex, which now belongs to Thermo Scientific. Thus, we offer the right HPLC column from Thermo for every need.
To find Thermo Scientific columns faster or to compare them with columns from other manufacturers, please use our column configurator. If you have any further questions, please do not hesitate to contact us personally.
Column description
Here you will find the brand name of the respective manufacturer for the column, e.g. "XBridge" columns from Waters. It is not necessarily recognizable which type of column the respective name refers to. If you have any questions, please do not hesitate to contact us.
Package specification
The packing specification describes the material of the stationary phase. The name indicates whether a functional group is bound to the carrier material (silica gel, polymer) and, if so, which functional group. Depending on the bound functional group (e.g. C18 chain), this results in the separation mode of the column (reversed phase).
Length
Depending on the type of application or system, different lengths of HPLC columns are used. The length of the column together with the internal diameter defines the column volume. For preparative separations, for example, columns from 250 mm in length with a larger internal diameter are often used; this allows more material to be separated in one run. If you have any questions about suitable HPLC column dimensions for your analysis, please do not hesitate to contact us.
Inner diameter
In addition to the length of the column, the inner diameter (ID) determines the column volume. The larger the internal diameter, the larger the volume and the higher the consumption of solvent. The inner diameter also influences the concentration of the analyte in the detector. For analyses where only a small amount of sample is available, it is advisable to use the smallest possible inner diameter. If an existing method is optimized from a larger to a smaller inner diameter, it must be ensured that the flow is adjusted for a comparable separation so that a constant linear velocity u is obtained
Particle Size
The choice of particle size for the column depends initially on the HPLC system used. Particle sizes <2µm can be used with a UHPLC. In an HPLC system, which can operate at pressures of up to 400 bar, particles of 5 µm are commonly used. The smaller the particle in the column, the higher the resulting back pressure in the HPLC system.
pH Range
HPLC columns have a certain pH stability. The manufacturer usually specifies a recommended pH value range for a particular column at which the column can be operated in the long term. A pH value >7 is considered critical for silica gels, as they gradually dissolve. This dissolution is slowed down by a special polymer coating or the introduction of hybrid material. If a pH value >8 is used during the routine, it is advisable to use a polymer-based material, for example.
Endcapping
In C18 columns, there are still free silanol groups on the carrier material between the bound C18 chains or other functional groups. These silanol groups interact strongly with alkaline molecules in particular, which can lead to an undesirable broadening of the peaks. In so-called endcapping, these silanol groups are usually "protected" with a trimethylsilyl group (TMS). The unwanted interaction and the resulting peak broadening can thus be suppressed.
Carbon load
The higher the percentage carbon level with the same packing density (g/ml), the higher the occupancy with e.g. C18 chains. The stationary phase is therefore more hydrophobic (non-polar). A non-polar sample molecule is therefore retained longer on a phase with a higher carbon content, which means that the retention time is slightly longer.
USP number
Monograph methods are frequently used, especially in the pharmaceutical industry. Users must then adhere to the requirements of the European Pharmacopeia (EP) or the US Pharmacopeia (USP), for example. The USP classification is based on the type of packaging material used. Classification "L1", for example, refers to an octadecylsilyl phase, better known as a C18 phase.
Phase Particle Size in µm Pore Size in Å Phase Particle Size in µm Pore Size in Å
120 C18 2.2, 3, 5
120
Phenyl 3
120
300 C18 2.2, 3, 5
300
Hilic 3
120
120 C8 2.2, 3, 5
120
Phase Particle Size in µm Pore Size in Å Phase Particle Size in µm Pore Size in Å
RP 2.6
80
PFP 2.6
80
C18 2.6
80
Phenyl-Hexyl 2.6
80
AQ 2.6
80
Hilic 2.6
80
Phase Particle Size in µm Pore Size in Å
C18 3, 5
100
Phase Particle Size in µm Pore Size in Å Phase Particle Size in µm Pore Size in Å
C18 3, 5
150
Phenyl 3, 5
150
C8 3, 5
150
CN 3, 5
150
C4 3, 5
150
Phase Particle Size in µm Pore Size in Å Phase Particle Size in µm Pore Size in Å
C18 3, 5, 10
100
Phenyl-Hexyl 3, 5
100
C8 3, 5, 10
100
CN 3, 5
100
C6 3, 5
100
Silica 3, 5
100
C1 5
100
Diol 5
100
Phenyl
100
Phase Particle Size in µm Pore Size in Å Phase Particle Size in µm Pore Size in A
C18 5
300
CN 5
300
C8 5
300
AX 5
300
C4 5
300
SCX 5
300
Phenyl 5
300
Phase Particle Size in µm Pore Size Phase Particle Size in µm Pore Size
C18 3, 5, 10
120
CN 5
120
C8 3, 5
80
Silica 3, 5
120
C4 3, 5
120
Amino 5
120
C1 3, 5
120
RP 3, 5
120
Phenyl 3, 5
120
CX 3, 5
120
Phase Particle Size in µm Pore Size in Å Phase Particle Size in µm Pore Size in Å
C18 2.4, 3, 5
130
Amino 1.9, 3, 5
175
C8 2.4, 3, 5
130
AX 1.9, 3, 5
175
Phenyl 2.4, 3, 5
130
SAX 1.9, 3, 5
175
Cyano 2.4, 3, 5
130
Silica 1.9, 3, 5
175
C18 Selectivity
1.9, 3, 5, 8, 12
175
PFP 1.9, 3, 5, 8, 12
175
C4 1.9, 3, 5
175
AQ 1.9, 3, 5, 8, 12
175
Phase Particle Size in µm Pore Size in A Phase Particle Size in µm Pore Size in Å
C18 3, 5, 8, 12
190
CN 5
190
C8 5
190
C4 3, 5, 8, 12
190
