Brownlee Analytical DB columns are moderately retentive HPLC and sub-2 μm UHPLC columns built on a foundation of high purity, base-deactivated silica that is optimized for UHPLC performance. These columns are available in 3 and 5 μm HPLC particle sizes, as well as a 1.9 μm UHPLC particle size. The silica manufacturing and phase bonding processes are identical for all particle sizes in this series resulting in method scalability from HPLC (3 and 5 μm) to UHPLC (1.9 μm), or vice versa. In addition, the larger 140 Å pore size allows analysis of a range of compounds from small molecules to short peptides while the base deactivation makes these columns appropriate for the analysis of acidic, neutral and basic compounds.