HPLC Column TSKgel Amide-80 HR, 80 Å Silica, 5 µm, 4.6 x 250 mm

The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction (HILIC) mode. Amide-80 shows higher retention of polar compounds than other amide phases. TSKgel Amide-80 columns packed with 2 μm silica based particles are the latest additions to the well-known TSKgel Amide-80 series. They are especially suited for use in UHPLC systems, as the reduced system volume and optimized detector specifications of UHPLC systems help to maintain the high resolution that can be achieved with 2 micron stationary phases.TSKgel Amide-80 can be operated over a temperature range of 10-80 °C (10-50 °C for Amide-80 2 & 3 μm). In general, retention times for carbohydrates decrease with increasing temperature. Below certain temperatures some carbohydrates may elute as split peaks. In this case, column heating or addition of triethylamine to the mobile phase is required. The pH range of mobile phase for TSKgel Amide-80 is 2.0-7.5 with a maximum salt concentration of 100 mmol/L. TSKgel Amide-80 is stable in 100% organic for normal phase separations; however, in HILIC mode the addition of water is necessary to create the water-rich surface layer.