Xtimate® SEC (size exclusion chromatography), also known as “global protein hydrophilic modified silica column”, is made from ultra-high purity, stable silica bonded with hydrophilic polymer and diol functional groups. This double bonding mechanism, which makes possible of nonspecific adsorption of high Mw polymers, proteins, biological enzymes, polypeptides and other biological samples, can be applied to separating water-soluble polymers from biomacromolecules.